INRA-ProbiHote

Team Interactions between commensal bacteria, probiotics and the host - Unité d'Ecologie et de Physiologie du Système Digestif

The aims are to understand how probiotics adapt to the digestive environment, define mechanisms involved and study functions of the microbiota in relation to diet-microbiota-host interactions with relevance to human nutrition and health.

Jean-Marc Chatel, PhD, worked for many years in the field of Immunology especially on the influence of antigen presentation on the immune response. Recently, he showed that transiting Lactic Acid Bacteria (LAB) were able to deliver fully functional plasmid to epithelial cells /in vivo/. He used these LAB's property to develop a novative way to prevent food allergy. Now, using the same approach, DNA delivery by LAB, he is focusing on the cross-talk between microbiota and gut epithelial cells.

Key persons involved

Philippe Langella, PhD lead the team working on the use of recombinant lactococci &  probiotics to deliver protein of interest.

3 Key publications

  • Sokol H , Pigneur B, Watterlot L , Lakhdari O, Bermudez-Humarán L, Gratadoux JJ, Blugeon S, Bridonneau C, Furet JP , Corthier G, Grangette C, Vasquez N, Pochart P, Trugnan G, Thomas G, Blottière HM, Dore J, Marteau P, Seksik P, Langella P. (2008) Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn's disease patients Proc Natl Acad Sci USA in press.[Pubmed]
  • Chatel JM, Pothelune L, Ah-Leung S, Corthier G, Wal JM, Langella P. (2008) In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells. Gene Ther. 15:1184-90.[Pubmed]
  • Bermudez-Humarán LG, Cortes-Perez NG, Lefèvre F, Guimarães V, Rabot S, Alcocer-Gonzalez JM, Gratadoux JJ, Rodriguez-Padilla C, Tamez-Guerra RS, Corthier G, Gruss A, Langella P. (2005) A novel mucosal vaccine based on live Lactococci expressing E7 antigen and IL-12 induces systemic and mucosal immune responses and protects mice against human papillomavirus type 16-induced tumors., J. Immunol.,175:7297-302.[Pubmed]