UO

Laboratory for Immunohistochemistry and Immunopathology (LIIPAT) - University of Oslo - Division of Pathology, Rikshospitalet - Oslo

The Laboratory for Immunohistochemistry and Immunopathology (LIIPAT) is located at the Institute of Pathology, Faculty of Medicine, University of Oslo, and the Division of Pathology, Oslo University Hospital - Rikshospitalet. Mucosal Immunology is the scientific focus of the group, which has collaboration with various clinical departments at Oslo University Hospitals and with several research institutions in Norway and abroad. LIIPAT is one the five groups that have joined to form the Centre for Immune Regulation (CIR) a Centre of Excellence selected by the Research Council of Norway, and a Federation of Clinical Immunology Societies (FOCIS) Centre of Excellence.

Key persons involved

Per Brandtzaeg, DDS, MS, PhD is the founder of LIIPAT. He has published more than 600 peer-reviewed original papers. He has received 14 awards all over the world. As a teacher, he has given ~300 contributions to postgraduate courses, ~680 invited lectures for various universities and societies, and supervised 40 doctoral dissertations.

 

Finn-Eirik Johansen, PhD is Prof. at UO. His research focus is the function of secretory antibodies in gut immunity and immune regulation in intestinal inflammation and allergy. He received his PhD from Columbia University (New York, NY, USA) in 1994.  He has published over 70 articles in peer-reviewed journals.

 

Research and Training Expertise

IgA (green) and IgG (red) staining in small intestine of polymeric Ig receptor (pIgR) knockout mouse (left) and wild type (right). Nuclei are stained blue with Hoechst dye. Note the absence of IgA staining in the epithelial crypt of pIgR-/- mice due to absence of pIgR mediated IgA transepithelial export.

LIIPAT is internationally recognized in mucosal immunology and inflammation and with its collaborators in CIR possesses all the necessary skills for biomedical research, from molecular and cell biology to live imaging. We have generated a knockout mouse that lacks secretory immunity (pIgR-/-); this strain is an interesting “tool” in studies of mucosal immunity–commensal interactions.

Material Ressources

Animal facility including transgenic centre and live imaging (Xenogen IVIS Spectrum), molecular biology including 4 colour real-time PCR, fluorescence microscopy including con-focal and live con-focal, electron microscopy, cell and tissue culture, FACS analysis and sorting.

There is available student accommodation (University housing) within walking distance from the hospital and other locations in the city of Oslo. Information can be found here.

Key publications

  • Brandtzaeg P. (2010) Food allergy: separating the science from the mythology. Nat Rev Gastroenterol Hepatol, 7:380-400. (Correction: Nat Rev Gastroenterol Hepatol, 2010;7:478)[Pubmed]
  • Wijburg OL, Uren TK, Simpfendorfer K, Johansen FE, Brandtzaeg P, Strugnell RA.(2006) Innate secretory antibodies protect against natural Salmonella typhimurium infection. J Exp Med., 203:21-6.[Pubmed]
  • Karlsson MR, Kahu H, Macpherson AJ, Johansen, F-E, Brandtzaeg P. (2010) Hypersensitivity and oral tolerance in the absence of a secretory immune system. Allergy, 65:561-70.[Pubmed]
  • Johansen FE, Pekna M, Norderhaug IN, Haneberg B, Hietala MA, Krajci P, Betsholtz C, Brandtzaeg P. (1999) Absence of epithelial IgA transport, with increased mucosal leakiness, in pIgR/SC-deficient mice. J Exp Med 190:915-21 [Pubmed]