RP10

PPAR-gamma and innate immunity to microbiota

For this research project, Mrs Agata KORECKA has been recruited.

Main supervisor

Karolinska Institute (S. Pettersson)

Co-supervisor

University of Debrecen (E. Rajnavolgyi)

Duration : 36 months 

Start date : end of 2008/beginning of 2009

Objective. To identify initiating maturation signals that may be a product of metabolic reactions, components of the microbiota, or innate immune cells.

Approach. The developmental stages of various tissues of newborn and 5 and 30 days old pups will be studied in GF and conventional mice by global gene expression profiling. The effect of the identified products on the expression, transcriptional activity and binding of PPARγ will be monitored in epithelial cells, macrophages and in DC subsets.

Deliverables:

  • Month-12 Gene expression profiling and identification of orthologues in human and other species
  • M-24 Validation by immuno-histochemistry and in situ hybridization, initial analysis in animal models or in cell lines by sRNAi
  • M-36 Identification of microbial compounds or strains involved, comparison of PPARγ-mediated responses in epithelial and phagocytic cells