RP8

Oral tolerance induction: impact of probiotics on epithelial and dendritic cells

For this research project, Mrs Elise HEUVELIN has been recruited.

Main supervisor

Nestlé (G. Prioult & A. Mercenier)

Co-supervisor

Istituto Europeo di Oncologia (M. Rescigno)

Duration : 24 months Post-Doc

Start date : end of 2008/beginning of 2009

Objectives. Determine the influence of probiotics on early markers of oral tolerance induction.

Approach. Co-incubation of intestinal cells (EC) from human gut tissue with probiotics and analysis of metabolites/molecules released by ECs by RT-PCR and metabolomics. Analysis of the ability of supernatant from probiotic-activated ECs to induce tolerogenic DCs in vitro. Implementation and use of a short mouse model of oral tolerance induction. In vivo modulation of oral tolerance induction by probiotics and correlation with in vitro results.

Expected impact: Identification of early markers of oral tolerance induction and better knowledge of the mechanisms by which probiotics may influence them (cross-talk with ECs and induction of tolerogenic DCs).

Deliverables:

  • Month-10: Impact of probiotics on epithelial cells and tolerogenic dendritic cells
  • M-12: Short model of oral tolerance induction established
  • M-24: Correlation between in vitro and in vivo results
  • M-30: publication