RP6

Effect of recombinant bacteria on the host immune system

For this research project, Mrs Marcella AZEVEDO has been recruited.

Main supervisor

INRA (P. Langella & J.M Chatel)

Co-supervisor

University of Wageningen (J. Wells)

Duration : 36 months 

Start date : end of 2008/beginning of 2009

Objective. Engineering of strains modulating immune response for vaccination and delivery of therapeutics.

Approach. Lactic acid bacteria (LAB) were shown to deliver cDNA in vitro in eukaryotic cells and in vivo in mice. Using reporter genes and imaging techniques, the uptake of LAB by DC and priming of T cells will be analyzed. The incidence of the nature of transiting LAB will be evaluated on the DNA translocation rate and immune response. These findings will be exploited to stimulate and analyze mucosal immune responses against the E7 Papillomavirus model antigen.

Deliverables.

  • M-10 Obtained and characterized the recLAB to be tested in vitro and in vivo
  • M-20 analysis of the fate of recLAB and of their interactions with DCs
  • M-30 evaluation of the selected recLAB in murine vaccination models
  • M-30 publications
  • M-36 PhD viva